Fig. 5. GFP-RabD^Q67L rings primarily enlarged lysosomes but not post-lysosomes. Both wild-type cells expressing GFP-RabD^wt and cells expressing GFP-RabD^Q67L (A-F) and RabD^Q67L (G-L) cells were pulsed with Texas Red dextran for 5 minutes and chased for 0 minutes to view macropinosomes (A,B,G,H); chased for 15 minutes to view lysosomes (C,D,I,J); and 60 minutes to view post-lysosomes (E,F,K,L). Cells were gently fixed and viewed using a fluorescence microscope using GFP and rhodamine filter sets. GFP-RabD(WT) is normally predominately localized to the contractile vacuole (B,D,F), whereas GFP-RabD^Q67L rings large vesicles (H,J,L) that accumulate fluid with the kinetics of lysosomes. Bar, 2 µm. The arrows in G and H reveals a macropinosome, whereas the arrows in I and J reveal enlarged lysosomes.

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