Fig. 7. RabD regulates phagocytosis in Dictyostelium Ax4 cells. In A, the rate of phagocytosis was measured using fluorescent latex beads (F-8816) as a phagocytic marker. Cells were shaken in suspension, and for each time point, 3x10⁶ cells were harvested, washed and lysed. Bead fluorescence was measured at an excitation of 625 nm and emission of 645 nm. B depicts the number of beads that were internalized at the 30 minute time point of the phagocytosis assay. Both mutants had statistically significant differences in the number of beads internalized compared with the control. (RabD^Q67L: P<0.0001; RabD^N121I: P<0.001).

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