Fig. 1. Inh2 localization corresponding to cell density. PC3 (A,B) and HeLa (C,D) cells were grown to either low (A,C) or high density (B,D) on fibronectin-coated coverslips and examined by immunofluorescence using an antibody against human Inh2. To confirm Inh2 distribution by western blotting, high-density (HD) or low-density (LD) HeLa cells were permeabilized with 0.01% digitonin to yield a cytosolic fraction, labeled C, and a nuclear fraction, labeled N. The top panel shows western blotting using an antibody to Inh2 and the bottom panel shows blotting with an antibody to RCC1 the Ran activator protein, a constitutively nuclear protein.

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