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Fig. 7. Inhibitor-2 is actively imported into the nucleus. A and B show low-density
HeLa cells expressing either an Inh2GFP3 fusion (A) or an
Inh2GFP3 fusion in which lysines 142 and 144 have been mutated to
alanine, termed NLS mutGFP3 (B). Cells were visualized with the
fluorescence of GFP and the nuclear, cytoplasmic and total fluorescence were
quantified. The graph shows the import index for GFP3,
Inh2GFP3 fusion, NLS MutGFP3 or
Inh2[Thr72Ala]GFP3 fusion.