Fig. 4. (A) The position of the nucleus in early meiotic prophase. The cell center-nucleus distance is the distance between the center of the cell and the center of the nucleus. A diagram of the distance () used to quantify nuclear displacement is shown. A box-whisker plot of the cell center-nucleus distance in pre-bouquet (n=12), bouquet (n=14) and post-bouquet (n=18) cells is shown. Distances were standardized to the nuclear radius. The cell center-nucleus distance units are based on the nuclear radius, such that the radius equals 1 unit. (B) Bouquet-stage polarization. The telomere-cell cortex angle is the angle created between the center of the telomeric heterochromatin, center of the nucleus and the center of the cell cortex. The cell cortex is the subset of the short side cell cortex bounded by tangents perpendicular to the nucleus. The telomere-NP angle is the angle created between the center of the telomeric heterochromatin, the center of the nucleus and the center of the NP-containing region. Diagrams of the telomere-cell cortex and telomere-NP angles used to determine bouquet-stage polarization are shown. Angles are indicated by . A box-whisker plot of telomere-cell cortex (n=22) and telomere-NP (n=30) angles in bouquet-stage cells is shown. Bouquet-stage angles were compared with the distribution of random angles between two points in a sphere through the center of the sphere (n=50). In both (A) and (B), the box represents the second and third quartiles, the horizontal line through the box is the median and whiskers extend to the range (see also Materials and Methods).

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