Fig. 7. Nuclear displacement and telomere positioning in colchicine-treated bouquet-stage cells. (A) Sequential z-axis projections of meiotic cells, as described in Fig. 3. Control and 250 µM colchicine-treated anthers were cultured for 14 hours. MTs (green) were detected with an antibody against -tubulin. Chromatin (red) was stained with DAPI, and telomere positions were inferred from the telomeric heterochromatin (a subset indicated with ^*). Bar, 10 µm. (B) Nuclear positioning in colchicine-treated cells. A box-whisker plot (as in Fig. 4) of telomere-cell center distance, as calculated in Fig. 4, for control (n=14) and 250 µM colchicine-treated (n=10) cells is shown. The cell center-nucleus distance units are standardized to the nuclear radius, such that the radius equals 1 unit. (C) Telomere polarization relative to the cell cortex in colchicine-treated cells. A box-whisker plot of telomere-cell cortex angle, as described in Fig. 6, for control (n=22) and 100 µM and 250 µM colchicine-treated (grouped together; n=22) cells, compared with the distribution of random angles in a sphere (n=50).

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