Fig. 3. GST-Mad1F10 can induce premature anaphase onset in prometaphase mammalian tissue cells. Prometaphase PtK1 cells were microinjected with either GST alone (top row) or GST-Mad1F10 (middle row). Microinjection occurred at the 0:00 time point for all rows. The injection of GST-Mad1F10 triggered premature anaphase onset prior to alignment of all chromosomes at the metaphase plate (middle row). Arrows mark unaligned chromosomes. GST-Mad1F10 also induced anaphase onset in cells pre-treated with 10 µM nocodazole to depolymerize all microtubules (bottom row). Asterisks indicates the injection needle. Bar, 5 µM.

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