Fig. 6. FRAP of tubulin-GFP. Maximum projections of confocal stacks of a scutellar bristle from a UAS-GFP-tubulin/+; neur-GAL4/+ pupa are shown. Prior to bleaching (A) the entire bristle shows strong fluorescence. The bristle immediately after bleaching is shown in B and the recovery of fluorescence is shown in micrographs taken at 5 minute intervals. Panels B, D and H also contain an insert showing the bleach/non-bleached boundary at a higher magnification. Shown below is a graph plotting fluorescence intensity as a function of time. The intensity was measured from the maximal projections (with no other manipulation) as locations approximately 5 µm proximal or distal to either the proximal or distal bleached/unbleached border. Note the gradual increase in fluorescence in the bleached region and the gradual decline of fluorescence in the unbleached region.

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