Fig. 2. SHIP2 was not tyrosine phosphorylated when plated on other ECM proteins. IPs and blots were carried out as described in Fig. 1. IPs were from HeLa cells that were adherent (Ad), detached (D) or re-plated (RP) for 60 minutes on surfaces coated with collagen I [6 µg/cm²; C.I, in the presence (+) or absence (-) of 1 µM Src inhibitor PD173956], fibronectin (5 µg/cm²; F), laminin (2 µg/cm²; L), collagen IV (6 µg/cm²; C.IV), or poly-L-lysine (0.01% solution, 0.5 ml/25cm²; P-Ly). Serum-starved adherent cells (Ad) treated with EGF (50 ng/ml for 5 minutes; E) or left untreated (control) are also shown. The arrow points to tyrosine-phosphorylated SHIP2.

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