Fig. 5. Confocal image analysis of a single differentiated P19 cell treated with KIF3A antisense ODN. (a-e) Differentiated untreated P19 cells. (f-j) KIF3A antisese ODN-treated cell. (k-o) KIF3A sense ODN-treated cell. (a,f,k) Localization of KIF3A protein, as visualized by staining with anti-KIF3A antibodies. (b,g,l) Expression of GFP-tau protein. (c,h,m) In situ hybridization with the GFP probe (which detects the transfected tau mRNA). (d,i,n) Localization of tubulin protein, as visualized by staining with tubulin antibodies. (e,j,o) In situ hybridization with tubulin. Solid arrowheads denote axons, empty arrowheads denote dendrites and small solid curved arrowheads denote neuronal cell bodies. Bar, 10 µm.

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