Fig. 7. Confocal image analysis of differentiated living P19 cell lines treated with emetine, a protein-synthesis inhibitor. (Aa,b) A control differentiated cell. The insert shows the enlargement of the growth cone region. (c,d) GFP-tau protein fluorescence in an emetine-treated cell. (e,f) GFP-tau protein fluorescence in a cell following a recovery period of 3 hours in fresh media. (B) Tau mRNA is found in emitine treated cells. (a) Phase view of a treated cell, which shows no GFP fluorescence (b). (c) In situ hybridization with GFP probe. Solid arrowheads denote axons, empty arrowheads denote dendrites and small solid curved arrowheads denote neuronal cell bodies. Bar, 10 µm.

Return to article