Fig. 6. Pharmacology and role of the K_Ca current on bone resorption. (a) 10 µM of the calcium ionophore A-23187 induced a reversible increase of the outward current (IK_Ca) that was completely and reversibly inhibited by 10 nM charybdotoxin (CTX) and partially blocked in an irreversible manner by 0.5 nM Apamin (Apa). (b) CTX and APA incubated during 24 hours with osteoclasts cultured on glass coverslips induced a significantly lower mean osteoclasts area (n70). (c) Bone resorption in vitro: CTX and APA were incubated during 24 hours with osteoclasts cultured on cortical bone slices. Both specific blockers of K_Ca channels inhibited the number of resorption pits compared with the control.

Return to article