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Fig. 3. Kinetics of GFP secretion from CHO cells. CHO cells transiently expressing
GFP were starved, metabolically labeled with 35S-methionine and
chased for various times as indicated. At each time point, the cell lysate (C)
and medium (M) were harvested and immunoprecipitated with anti-GFP antibodies
and analyzed by SDS-PAGE followed by fluorography. The graph depicts the
percentage of secretion at each time point.
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