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Fig. 4. GFP secretion is a temperature-dependent process. CHO cells transiently expressing GFP were starved, labeled with [35S]-methionine at 37°C and chased for 6 hours at various temperatures as indicated above each set of lanes. The cell lysates (C) and media (M) were harvested and immunoprecipitated with anti-GFP antibodies. `% Sec.' denotes the percentage of secretion.





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