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Fig. 6. Effect of overexpression of Gcs1p on mitochondrial morphology does not require its zinc-finger motif. Data are presented as in Fig. 3. Upper panel, lysates of spheroplasts from gcs1 cells overexpressing Gcs1{Delta}Zn and grown in minimal medium were fractionated for analysis by immunoblotting. Lower panel, indirect immunofluorescence staining of gcs1 cells overexpressing Gcs1{Delta}Zn. Spheroplasts were reacted with purified anti-Gcs1p antibody (B) or anti-porin antibody (C) or stained with H33258 (A). Arrows indicate tubular mitochondria and arrowheads mitochondria.





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