Fig. 1. Effect of expression of NCAM-140 on single-cell motility of glioma cells. (A) Phase-contrast image of glioma cells plated as single cells on fibronectin and transiently transfected with plasmids encoding NCAM-140 and EGFP overlaid with the fluorescence image of the EGFP-expressing cells. (B) ‘Wind-rose’ plots of tracks with superimposed starting points of glioma cells transiently transfected with a plasmid encoding EGFP and either co-transfected with a plasmid encoding NCAM-140 or a control plasmid. The circles mark the square root of the mean-squared displacement (
) of the recorded cells. (C) Cell motility measured as rate of diffusion, R; (D) root mean square speed, S, and (E) mean cell speed, S
, of glioma cells transiently transfected with vector (control) or a plasmid encoding NCAM-140 (the mean from five experiments was *P<0.05, **P<0.01 using student’s paired t-test). (F,G) The distribution of the S for populations of cells from representative experiments of cells transiently and stably transfected with a control plasmid (F) or a plasmid encoding NCAM-140 (G).
