Fig. 6. There are low levels of some proteins (Bud3p, Bud4p and Spa2p) in chs2 and myo1 mutants (A). In (B), the decrease in level of Bud4p, when fused to GFP, is much less than with the native protein. The strains used are YPH499 (wild-type), YMS11 (chs2
), YMS57 (myo1), YMS160 (Bud4p-GFP), YMS164 (chs2, Bud4p-GFP) and YMS166 (myo1, Bud4p-GFP). For the detection of Spa2p-HA and Myo1p-c-myc, YPH499 containing pMS53 or pMS54, respectively, was used. Hsp104p was detected as a loading control. In this figure, relevant genotypes are listed above the blot and detected proteins on the right side of it. For the western blotting technique used see Materials and Methods.
