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Fig. 3. Intracellular compartments containing internalized EGF lack accessible PtdIns(4,5)P2. The pleckstrin homology domain of PLC{delta}1, which is fused to enhanced GFP (GFP-PH), was used as a probe to visualize PtdIns(4,5)P2. GFP-PH-transfected NR6 WT cells were either (A) untreated or (B) stimulated with EGF-TR for 20 minutes. Cells were visualized by confocal fluorescence microscopy. (C) GFP-PH-transfected, EGF-TR-treated parental NR6 cells were used as a control. Scale bar, 10 µm.





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