Fig. 3. Effect of IBMX and forskolin on sperm-induced GVBD. Oocytes were either left untreated (, Control) or pre-incubated in presence of 65 µM IBMX and 60 µM forskolin (, Forsk/IBMX) or an equivalent amount of DMSO (, DMSO), for 15 minutes prior to insemination at time t=0. Aliquots were sampled at indicated times for determination of percentage GVBD. Mean results (±s.e.m.) of two separate experiments are shown.

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