Fig. 1. IdPCR and ddPCR analysis of ES and EPL cell RNA. (A) Cytoplasmic RNA was isolated from ES cells and EPL cells grown for 4 or 6 days in the presence of 50% MEDII + LIF (+). Then, 2 µg of this RNA were analysed by idPCR using the 5' primer OPB-04 (GGACTGGAGT; Operon Technologies). PCR products were resolved by electrophoresis on a 6% (v/v) denaturing polyacrylamide gel. (B) Cytoplasmic RNA was isolated from ES cells and EPL cells grown for 2, 4 or 6 days in 50% MEDII in the presence (+) or absence (–) of LIF. Then, 2 µg of this RNA were analysed by ddPCR using the 5' primer OPA-01 (CAGGCCCTTC; Operon Technologies) PCR products were resolved by electrophoresis on a 6% (v/v) denaturing polyacrylamide gel. Arrows mark the indicated products.
