Fig. 5. The localisation of endogenous lamin A/C and exogenously expressed EGFP-emerin in the nucleus after cell division. A series of z-sections (0.2 µm apart) were taken through the nuclei of the transfected COS-7 cells in interphase 28 hours after being released from nocodazole treatment. EGFP-emerin is shown in green and endogenous lamin A/C shown in red. The lamin A/C localisation was unaffected in cells expressing wild-type EGFP-emerin (A; 18 z-sections taken in total); however, in the cells expressing the mutant forms of emerin, endogenous lamin A/C was redistributed. The nuclear morphology was altered in the cells transfected with Del95-99 (B; 23 z-sections taken in total) and those cells with irregularly shaped nuclei consistently had exogenously expressed Del95-99 EGFP-emerin and endogenously expressed lamin A/C lost from one of the poles. The nuclear morphology was also altered in cells that expressed Del236-241 (D; 31 z-sections taken in total) EGFP-emerin, and the number of z-sections show that the cells expressing this mutant were smaller and rounder. Intranuclear tubules of emerin and lamin A/C are seen to a greater extent in cells transfected with either wild-type (A) or missense mutations (C; S54F). Radiance Confocal Laser Scanning System; bar, 5 µm.
