Fig. 6. A number of COS-7 and HeLa cells transfected with either wild-type or mutant EGFP-emerins show endogenous lamin A/C redistribution and altered nuclear morphology. The endogenous lamin A/C and nuclear architecture appeared unaffected (100%) in both COS-7 or HeLa cells transfected with wild-type (wt) EGFP-emerin (COS-7; n=3, an average of 857 cells per count, HeLa; n=3, an average of 691 cells per count). The number of cells transfected with either Del236-241 (COS-7; n=3, an average of 595 cells per count, HeLa; n=3, an average of 124 cells per count) or Del95-99 (COS-7; n=3, an average of 1169 cells per count, HeLa; n=3, an average of 852 cells per count) EGFP-emerin that had irregularly shaped nuclei with lamin A/C mislocalised was greater than the number of cells that had round nuclei with lamin A/C localised. The percentage of cells expressing S54F EGFP-emerin (COS-7; n=3, an average of 1204 cells per count; HeLa; n=3, an average of 713 cells per count) that had either irregularly shaped nuclei with lamin A/C mislocalised or round-shaped nuclei with lamin A/C localised was similar. The quantification data show that, after cell division, the redistribution of lamin A/C depends upon the localisation of EGFP-emerin.
