Fig. 1. (A) Western blot developed using an anti-galectin-1 antibody. Lane 1, molecular weight markers; lanes 2 and 4, no samples were run; lane 3, galectin-1 positive control; lane 5, C2C12-conditioned media; lane 6, media from COS-1 cells transfected with the galectin-1 construct; lane 7, the non-transfected COS-1 media. The positive galectin-1 band is at approximately 14.5 kDa in lanes 3, 5 and 6. (B) Dot-blot developed using an anti-galectin-1 antibody. Samples 1, 2 and 3 are purified recombinant galectin-1 (1.85 mg/ml) diluted 1:10, 1:50 and 1:100, respectively. Sample 4 is non-diluted GAL-M.
