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Fig. 3. C2C12 cells grown in FCS-20+GAL-M after 2-3 days of growth. Cells stain positively for the muscle-specific marker desmin. A myotube with three nuclei is evident (A). All cells stain positively for vimentin, a mesenchymal cell marker (B) and terminally differentiated cells are positive for myosin II heavy-chain (C). Nuclei are labelled with DAPI in B and C. Bars, 50 µm.





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