Fig. 2. Regulated expression of the scaffold protein IB1/JIP-1. (A) Northern blot analysis of bladders revealed that the level of IB1/JIP-1 transcripts is drastically decreased in dilated bladder after seven hours of dilation. In the same samples, levels of GAPDH mRNA were slightly increased (1.5 fold) in the dilated bladder. Each lane is from a different animal and was loaded with 10 µg mRNA. (B) Quantitative assessment of sixteen experiments (one per rat) confirmed that the levels of IB1/JIP-1 mRNA were decreased by three fold compared with control values. Values represent ratios of densitometric measurements of IB1/JIP-1 and GAPDH mRNAs and are expressed as mean±s.e.m. (n=16± s.e.m.; *P<0.01). (C) IB1/JIP-1 content was reduced in dilated bladder after seven hours of dilation as assessed by western blot analysis. Equal protein loading was evaluated by measuring the level of tubulin. (D) Quantitative evaluation of four immunoblots revealed that the levels of IB1/JIP-1 were reduced by 50% in samples of dilated bladder (seven hours after ligation) compared with their controls. Values represent ratios of densitometric measurements of IB1/JIP-1 and tubulin. (mean±s.e.m.; n=5; P<0.05).
