Fig. 2. Rho-GTPase-inactivation inhibits CD82-induced morphological changes. (a,b) Untreated Jurkat cells (a) and Jurkat cells treated (b) for two hours with 0.1 µg/ml of Toxin B from C. difficile were cultured for one hour at 37°C, on anti-CD82-coated plates (
C11, 50 µg/ml). (c,d) Jurkat cells were cultured at 37°C on anti-CD82-coated plates (C11, 50 µg/ml). After one hour, 0.1 µg/ml of Toxin B was added (d), or not (c), to the culture medium and the cells were cultured for another two hours on anti-CD82 coated plates. After removing non-adherent cells, Jurkat cells were fixed, permeabilized and stained with rhodamine-phalloidin. The data shown are representative of five independent experiments. Bar represents 10 µm.
