Fig. 5. CD82-specific translocation to the detergent insoluble fraction is independent of Rho GTPases. Jurkat cells, treated with 0.1 µg/ml Toxin B for two hours or untreated, were cultured for 15 minutes at 37°C on antibody-coated plates (anti-CD82: 
c11, 50 µg/ml; anti-CD9:Syb1, 50 µg/ml; anti-CD81: Z81, 50 µg/ml). After SDS-PAGE analysis, proteins of the soluble (equivalent to 105 cells per lane) and insoluble fractions (equivalent to 2x106 cells per lane) were probed by immunoblotting with anti-CD82 or anti-CD81 mAbs. (A) Stimulation: lane 1, 0; lane 2, anti-CD82; lane 3, anti-CD9; lane 4, anti-CD81. The left and right panels represent the soluble and insoluble fractions respectively. The lower and upper panels represent western blots with anti-CD82 and anti-CD81 antibodies, respectively. (B) Stimulation: lanes 1-2, no mAb; lanes 3-4, anti-CD82 mAbs (C11, 50 µg/ml). Treatment: lanes 1, 3, no addition; lanes 2, 4, 0.1 µg/ml Toxin B. Left panel and right panels represent soluble and insoluble fractions, respectively. The data are representative of three independent experiments.
