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Fig. 3. ATPase activity of myosin VIIa. (A) A histogram illustrating the dependence of Mg2+ (2 mM) and actin filaments (2.5 µM) on myosin VIIa ATPase activity. (B) Relationship between myosin VIIa ATPase activity and the concentration of actin filaments. From these data a maximal ATPase activity (Kcat) of 4.3 s–1, with 7.0 µM of f-actin required for half-maximal activity (Kactin), was calculated.





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