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Fig. 4. {Delta}vps30 and {Delta}vps38 mutant cells exhibit defects in PtdIns3P synthesis. Wild-type (SEY6210), {Delta}vps30 (JCY300), {Delta}vps38 (PBY58), and {Delta}vps30{Delta}vps38 (PBY51) cells were grown in YNB to early log phase and labeled with myo-[2-3H] inositol at 26°C for 45 minutes. Cellular lipids were recovered, deacylated and separated by HPLC. Levels of deacylated products corresponding to the indicated phosphoinositides are shown. These data represent the means±s.e.m. of at least three independent experiments. PtdIns3P, phosphatidylinositol 3-phosphate; PtdIns(4)P, phosphatidylinositol 4-phosphate; PtdIns(3,5)P2, phosphatidylinositol (3,5)-bisphosphate; PtdIns(4,5)P2, phosphatidylinositol (4,5)-bisphosphate.





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