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Fig. 5. Recruitment of Vps5p and Vps17p to the endosomal membrane depends on the synthesis of PtdIns3P by the Vps34p PtdIns 3-kinase complex II. (A) Localization of Vps5-GFP in wild-type (PBY86), {Delta}vps34 (PBY88) and {Delta}vps38 (PBY87) cells (left panel) and Vps17-GFP in wild-type (PBY70), {Delta}vps34 (PBY111) and {Delta}vps38 (PBY112) cells (right panel) by fluorescence microscopy. (B) Fluorescence and DIC microscopy of Vam7-GFP (pTKS35) expressed in wild-type (SEY6210) and {Delta}vps38 (PBY58) cells, respectively. (C) Fluorescence and DIC microscopy of vps34ts cells expressing Vps17-GFP (PBY123). The cells were incubated at non-permissive temperature (37°C) as indicated.





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