Fig. 2. Characterization and isolation of the mAb H22 antigen. (A-C) 2D electrophoresis of capsule proteins according to pI and molecular weight. The protein of two million capsules was separated and analyzed by Coomassie staining (A), western analysis with mAb H22 (B), and silver-staining (C). (D) Assay of N-glycosylation. Solubilized capsule protein was incubated with N-glycosidase F to remove N-linked sugars. The deglycosylated protein and mock control (without enzyme) were separated by SDS-PAGE and probed with mAb H22 (left) and anti-CTLD antibody (right) in western analysis.

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