Fig. 1. Paramecium NSF genes. (A) Simplified restriction map of PtNSF1 and PtNSF2 genes and position of the probes and oligonucleotides used in this study. (B) Southern blot analysis of wild-type genomic DNA probed with the long probe (primers 3-4). A star marks the bands also revealed by the short probe (primers 1-2). Bc, BclI; Bg: BglII; E, EcoRV; S, SwaI; X, XbaI. (C) Northern blot analysis of total RNA from the wild-type (WT) and from the nd9-1 mutant (1) altered in the terminal step of exocytosis. Three probes were used, the long PtNSF probe, and, as controls, probes specific for the ND9 and ND7 genes (Froissard et al., 2001). By using the long PtNSF probe, a 2.3 kb band is revealed in both strains, with similar intensity.

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