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Fig. 2. Multiple alignment of NSF genes from different species. NSF sequences from Paramecium tetraurelia (PtNSF1 and PtNSF2), Rattus norvegicus (P18708), Arabdidopsis thaliana (AAD17345), Dictyostelium discoideum (AAC48226) and Saccharomyces cerevisiae (AAA35031) were aligned using the CLUSTALW program and displayed using BOXSHADE. The conserved AAA domains of NSF ATPases are underlined. The Walker A and B domains (Patel and Latterich, 1998) are boxed in red. The limits between the N and D1, and D1 and D2 domains are indicated by vertical bars. An SRH domain is boxed in green and a divergent SRH domain is also boxed in green with dotted lines.





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