Fig. 5. (A) PCR analysis shows that cells from the follicle dermis reconstitute bone marrow, spleen and peripheral blood of recipients. The lacZ gene was detected in the peripheral blood, bone marrow and spleen of the Zin40 donor mouse, but not in the control recipients (Balb/c). M15 and M16 were transplanted with 2x10⁴ Zin40 dermal sheath cultured cells and 2x10⁵ Balb/c bone marrow cells. M18 and M28 were transplanted with 2x10⁴ Zin40 dermal papilla cultured cells and 2x10⁵ Balb/c bone marrow cells. NC, no DNA control. (B) Dermal-derived cells contribute to all haematopoietic lineages studied: confirmation by PCR. (A) Splenocytes from Balb/c recipient animals transplanted with dermal cells derived from LacZ transgenic animals (Zin40) were sorted into subpopulations (total leukocytes, CD45+; non-haematopoietic nucleated cells, CD45-; B cells, B220+; T cells, CD4+/CD8a+; and myeloid cells, CD11b and Ly6) and presence of transgenic leukocytes determined by PCR. A second PCR was performed with Wnt8b primers as a loading control. (C) Detection of transgenic donor cells by PCR. Mixtures of splenocytes from transgenic and non-transgenic animals were mixed in differing ratios from 100% transgenic to 0% transgenic and subjected to PCR for LacZ and Wnt8b.

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