Fig. 5. HDLp colocalizes with internalized Tf in the ERC. Directly after preincubation for 20 minutes at 18°C with OG-HDLp and TMR-Tf, iLR-transfected CHO cells were rinsed in HEPES buffer and mounted in an aluminium chamber. The living cells were observed at 37°C in chase medium and imaged using confocal laser scanning microscopy. Digital multicolour images of OG-HDLp (left panel) and TMR-Tf (middle panel) were taken at defined time points as indicated in the left panel. Colocalization was visualized by merging the two images of the same time point (right panel). The insert in the upper right panel shows a bright-field image of the observed cells directly after preincubation. Bar, 20 µm.

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