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Fig. 8. HDLp exits CHO(iLR) cells with a t
of 
13 minutes. Living
CHO(iLR) cells, preincubated with DiI-LDL and OG-HDLp, were observed in an
aluminium chamber with confocal laser microscopy during a chase for 20
minutes. Digital photos were collected 10 minutes after initiating the chase
at 2 minute time intervals (A-F) to visualize the intracellular distribution
of LDL (red) and HDLp (green). To quantify the re-secretion of internalized
fluorescently-labeled lipoproteins, CHO(iLR) cells were fixed directly after
preincubation with DiI-LDL (filled circles) or OG-HDLp (open circles), and
after a chase for 10, 30 or 60 minutes (G). Images were recorded using
confocal laser scanning microscopy and analyzed with Scion Image software. The
data points are geometric means of the measured relative intensity of total
cells. Of each time point, 10 to 17 images were taken with 4 to 33 cells per
image, corresponding to more than 200 cells per data point. The mean values
were normalized to the measurement directly after the preincubation. The data
were processed with Microsoft Excel and plotted with SigmaPlot. Bar, 20 µm;
values are means±s.e.m. of 10 or more digital images.
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