Fig. 2. Alignment of UCS domains from fungal homologues and animal UNC-45 proteins. Only one UCS protein is present in fungi and invertebrates. Two UNC-45 isoforms, SM (striated muscle) and GC (general cell) are present in the bony fish Fugu rubripies, suggesting that the gene divergence is ancient. Note that the fungal proteins have extra residues in five places, compared with the animal proteins. Identity is indicated by black boxes, similarity by grey boxes. There is a clear distinction between invertebrate and vertebrate proteins. Blue arrowheads indicate completely conserved residues, and red arrowheads indicate 90-95% identity. Asterisks indicate conserved residues corresponding to C. elegans temperature-sensitive UNC-45 (Barral et al., 1998) and S. pombe Rng3p mutations (Wong et al., 2000). Ano. gam., Anopheles gambiae (coding sequence compiled from acc. no. AAAB01008844.1); Dros. melan., Drosophila melanogaster (acc. no. AAK93568); C. elegans, Caenorhabditis elegans [(Venolia et al., 1999) acc. no. AAD01976]; C. briggsae, Caenorhabditis briggsae [(Venolia et al., 1999) acc. no. AAD01960]; Daniorerio, Danio rerio (acc. no. AAL57031); Fugurub., Fugu rubripies (SM, coding sequence compiled from JGI Fugu genome project scaffold 6404; GC, coding sequence compiled from JGI Fugu genome project scaffold 465); Human, Homo sapiens (SM, coding sequence compiled from AC022916; GC, acc. nos. BAB20273, AAH06214); N.c., Neuropsora crassa (direct submission acc. no. T49461); Mouse, Mus musculus (SM, coding sequence compiled from acc. no. AL603745; GC, acc. no. AAH04717); P.a., Podospora anserina [(Berteaux-Lecellier et al., 1998) acc. no. CAA76144]; S.c., Saccharomyces cerevisiae [(Jansen et al., 1996) acc. no. CAA63795]; S.p., Schizosaccharomyces pombe [(Wong et al., 2000) acc. no. O74994], Xen. laev., Xenopus laevis (coding sequence compiled from acc. nos. AW765658, BJ074261 and BJ091725), Xen. trop., Xenopus tropicalis (coding sequence compiled from acc. nos. AL650279, AL656414, AL661438 and AL646713).

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