spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Expression of wild-type as well as mutant human PrP and wild-type bovine PrP in transfected BHK cells and the effect of N-glycosidase F and endoglycosidase H. Immunoblot analysis of prion proteins from total cell lysates of transfected BHK cells. Cells were transiently transfected with recombinant SFV RNAs encoding wild-type human PrP (A, lanes 1 and 2; B, lanes 3 and 4), human PrP-FFI (A, lanes 3 and 4; B, lanes 5 and 6), human PrP+9OR (A, lanes 5 and 6; B, lanes 7 and 8) or wild-type bovine PrP (A, lanes 7 and 8) and with SFV-1 RNA (without any insert) (B, lanes 1 and 2). 24 hours post-transfection the expression was analyzed with mAb anti-PrP 3B5. Glycosylation patterns: di-, mono- and non-glycosylated PrP isoforms. (A) Analysis of prion proteins huPrP (lane 2), huPrP-FFI (lane 4), huPrP+9OR (lane 6) and boPrP (lane 8) after N-glycosidase F treatment. (B) Analysis of prion proteins huPrP (lane 4), huPrP-FFI (lane 6) and huPrP+9OR (lane 8) and cellular proteins from SFV-1-transfected cells (lane 2) after endoglycosidase H treatment.





Right arrow Return to article