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Fig. 5. Accumulation of His-tagged GFP (AtpC4-His) in the central region of the plastid in P. tricornutum. (A) Top, GFP fluorescence; bottom, superimposed GFP and chlorophyll fluorescence (the red chlorophyll fluorescence marks the two lobes of the plastids, P). The fusion protein appears to accumulate between the two plastid lobes. (B) Electron micrograph of a P. tricornutum cell immuno-decorated with antisera against GFP. GFP accumulates in the central region of the plastid (arrow), where two plastid lobes are connected. Py, pyrenoid; M, mitochondrium; N, nucleus; V, vacuole. Bars, 5 µm (A); 0.2 µm (B).





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