Fig. 5. Quantitation of PLP and IRBP gene nuclear localization. The PLP and IRBP genes were classified as within the peripheral region of the nucleus if the measured distance between the center of the in situ hybridization signal and the edge of the nucleus was less than 1.5 µm. When the center of the in situ hybridization signal was located within the remaining nuclear volume, the localization was counted as central. In panel A, phase-contrast microscopy was used to identify the nuclear boundary. The PLP gene was more frequently associated with the peripheral region of the nucleus in oligodendrocyte progenitors (OPs, n=22) and oligodendrocytes (oligos, n=37). Analysis of individual IRBP alleles within each cell did not indicate a preferential association of IRBP alleles with the peripheral region of the nucleus (OPs, n=38; oligos, n=30). In panel B, both IRBP alleles were analyzed within each cell and were categorized as central-central (CC), central-peripheral (CP), or peripheral-peripheral (PP). In panel C, DAPI was used to identify the nuclear periphery. The PLP gene was non-randomly associated with a peripheral localization in astrocytes (astros, n=52), oligodendrocyte progenitors (OPs, n=103), or oligodendrocytes (oligos, n=101) (^*P<0.05, chi-square). The PLP gene localization was not significantly different when comparing across cell types. Error bars=standard error of the proportion.

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