Fig. 7. Bgs1p localization during the sexual phase of the life cycle suggests that it is involved in mating projection synthesis, cell fusion control and forespore and spore wall synthesis. Homothallic GFP-bgs1⁺ bgs1 h⁹⁰ cells grown at 28°C in EMM liquid medium until early-stationary phase were collected, transferred onto a SPA plate and incubated at 28°C. Samples were taken after 3, 5, 8, 24 and 48 hours, resuspended in EMM liquid medium containing 50 µg/ml Calcofluor white, and visualized for GFP and Calcofluor white staining. Cells and zygotes representative of each mating and sporulation step were selected and aligned to show a sexual phase progression.

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