Fig. 8. Bgs1p localizes around the spore during isotropic growth, to the growing pole during spore germination, and to the contractile ring and septum during cytokinesis of the first cell. Homothallic GFP-bgs1⁺ bgs1 h⁹⁰ strain was grown and sporulated as in Fig. 7. Cells were incubated on SPA plates at 28°C for 7 days to allow most of the asci to spontaneously lyse and liberate the spores. The culture was checked to confirm that most of the spores were released. The spores were collected, incubated in YES liquid medium at 28°C, and samples were taken at 5 and 9 hours. Calcofluor white was added (50 µg/ml final concentration) and the spores were examined for GFP and Calcofluor white staining. Spores representative of each germination step were selected and aligned to show an ordered germination process.

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