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Fig. 3. Analysis of sex body formation and disappearance in wild type (a-c), Xisttrun (a'-c') and Xist1lox (a''-c'') spermatocytes using the sex body marker {gamma}-H2AX. ({gamma}-H2AX, green; SYCP3, red; X chromosome, short arrow; Y chromosome, arrowhead). {gamma}-H2AX labels the sex chromosomes prior to their incorporation into the sex body (a,a',a'') and then associates with the sex body throughout pachytene (b,b',b''). It finally disappears as sex bodies are disassembled, at metaphase I (c,c',c''). Bar, 10 µm.





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