Fig. 7. Analysis of MSCI by Rbmy-inactivation in wild type (a-f), Xist^trun (a'-f') and Xist^1lox (a''-f'') spermatogenic cells (RBMY, red; XMR, green; forming sex body, black arrowhead, mature sex body, white arrowhead). Spermatogonia, identified by their lack of staining for XMR (a,a',a'') abundantly express RBMY (b,b',b''). Late zygotene spermatocytes, identified by their low level nuclear XMR staining with brighter superimposed sex chromosome XMR staining (c,c',c''), express RBMY at lower levels (d,d',d''). Pachytene spermatocytes, identified by their mature XMR-positive sex bodies and absent whole nuclear XMR staining (e,e',e'') contain undetectable levels of RBMY (f,f',f'') in all three genotypes. Bar, 5 µm.

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