Fig. 2. Characterization of the erythroid ankyrin-associated kinase using an in gel kinase assay. A whole cell lysate was prepared from erythroid cells isolated from 10-day-old chicken embryos. This lysate (lanes 1 and 2) and an ankyrin immunoprecipitate prepared from the lysate (lane 3) were electrophoresed on a 12.5% SDS polyacrylamide gel containing either 50 µg/ml myelin basic protein (lane 1) or 50 µg/ml of chicken red blood cell membranes (lanes 2 and 3). Following electrophoresis, the gels were processed as described in the Materials and Methods and incubated in kinase buffer containing 25 µM ATP and 100 µCi [-³²P] ATP. The gel was then washed and dried, and ³²P-labeled species were detected by autoradiography. Molecular weight markers are indicated to the left of the figure.

Return to article