Fig. 4. Overexpression of LGP85 does not cause enlargement of lysosome, but results in formation and accumulation of the late endosome-lysosome hybrid organelle. To label lysosomes, COS cells were incubated with Texas-Red dextran for 4 hours and chased for 20 hours. After that, cells were transiently transfected with LGP85 and fixed at 12 (A-C), 24 (D-F), and 36 (G-I) hours after transfection, followed by staining for LGP85 (A,D,G, green). Cells were visualized by confocal microscopy. The right columns show the merged images of LGP85 (green) and dextran (red). Bars, 20 µm.

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