Time-lapse fluorescence microscopy depicting the distribution of labelled CKs in a hepatocellular-carcinoma-derived PLC clone PK18-5 stably expressing fluorescent fusion protein HK18-YFP. The inverse contrast images were recorded every second and demonstrate details of CKF-disruption starting 234 seconds after addition of OV (50 mM) (see also Fig. 9).

Inverse fluorescence microscopy of living OV-treated (10 mM) AK 13-1 cells detecting HK13-EGFP. Images were recorded every 8 seconds and are presented as averages of consecutive frames. Fine granules are formed within filaments reaching a maximum 12 minutes after OV addition and gradually re-integrating into CKFs, with almost no thickenings remaining, by 38 minutes (see also Fig. 10).

High magnification of a region taken from Movie 3 showing the distribution of HK13-EGFP in AK13-1 cells after a 3 minute treatment with 10 mM OV (recording intervals 15 seconds). For best visualization, the pictures are shown as averaged consecutive frames. Note the elongation and fusion of granules and their integration into a fine CKF network.