Fig. 7. Time-lapse fluorescence microscopy of AK13-1 cells depicting the altering distribution of HK13-EGFP fluorescence after OV addition (10 mM). The inverse contrast images are taken from Movie 2 (recording intervals 8 seconds; available at jcs.biologists.org/supplemental). Note the formation of fine granules within filaments reaching a maximum 12 minutes after OV addition and the gradual re-integration of these granules into CKFs with almost no thickenings remaining by 38 minutes (arrows). Separating desmosome-anchored filaments are demarcated by arrowheads. Bar, 10 µm.

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