Fig. 8. Fluorescence micrographs taken from a time-lapse recording of AK13-1 cells monitoring the distribution of fusion protein HK13-EGFP following a transient 3 minute treatment with 10 mM OV. Note the reformation of an extended CKF network within less than 30 minutes with no remaining granules and a homogenous composition. The entire sequence is provided as Movie 3 (recording intervals 15 seconds; available at jcs.biologists.org/supplemental). Elongation and fusion of granules and their integration into a fine network is shown at high magnification in the lower panel (corresponding to the boxed area in upper panel). The dynamics of this process can be best appreciated in the corresponding Movie 4. Bar, 10 µm in upper panels and 1 µm in lower panels.

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