Fig. 6. MAPK signalling in pig thyrocytes treated with EGF and TGF-ß1. (A) Phosphorylation of Erk by TGF-ß1 or EGF. Cells were stimulated with TGF-ß1 (10 ng/ml) or EGF (10 ng/ml) for 15-45 minutes and then solubilised. Proteins were detected by antibodies against phosphorylated Erk (P-Erk 1/2) and total Erk (Erk 1/2). Note diminutive amounts of Erk phosphorylation in TGF-ß1-treated cells only after 15 minutes. (B) Effect of TGF-ß1 on EGF-induced Erk phosphorylation. Cells were exposed to EGF in the presence or absence of TGF-ß1 for the indicated times and then analysed for the presence of phosphorylated Erk and total Erk as outlined in A.

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